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recombinant mouse trail protein rmtrail  (PeproTech)


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    Structured Review

    PeproTech recombinant mouse trail protein rmtrail
    ILz:rhTRAIL showed the highest cell death inducing ability in <t>TRAIL</t> susceptible cells. Indicated cells were cultured onto a 96-well plate and treated with <t>varying</t> <t>recombinant</t> TRAIL proteins: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL. ( a ) HeLa, CT26, and B16F10 cells were treated with varying recombinant TRAIL proteins (100 ng/ml). After 16 h, cell survival was examined by XTT assay. ( b ) Three human cell lines (Jurkat, MDA-MB-231, and HEK 293) and two murine cell lines (BMK and 4 T1) were treated with varying recombinant TRAIL proteins (100 ng/ml). To investigate TRAIL-susceptibility, cell death was analyzed by XTT assay 24 h after treatment. The relative values of the XTT assay were examined after comparing the results to untreated controls. * p < 0.05, compared with untreated controls by Student’s t -test
    Recombinant Mouse Trail Protein Rmtrail, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+trail+protein+rmtrail/pmc05902847-58-3-12?v=PeproTech
    Average 90 stars, based on 1 article reviews
    recombinant mouse trail protein rmtrail - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "The regulation of combined treatment-induced cell death with recombinant TRAIL and bortezomib through TRAIL signaling in TRAIL-resistant cells"

    Article Title: The regulation of combined treatment-induced cell death with recombinant TRAIL and bortezomib through TRAIL signaling in TRAIL-resistant cells

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4352-3

    ILz:rhTRAIL showed the highest cell death inducing ability in TRAIL susceptible cells. Indicated cells were cultured onto a 96-well plate and treated with varying recombinant TRAIL proteins: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL. ( a ) HeLa, CT26, and B16F10 cells were treated with varying recombinant TRAIL proteins (100 ng/ml). After 16 h, cell survival was examined by XTT assay. ( b ) Three human cell lines (Jurkat, MDA-MB-231, and HEK 293) and two murine cell lines (BMK and 4 T1) were treated with varying recombinant TRAIL proteins (100 ng/ml). To investigate TRAIL-susceptibility, cell death was analyzed by XTT assay 24 h after treatment. The relative values of the XTT assay were examined after comparing the results to untreated controls. * p < 0.05, compared with untreated controls by Student’s t -test
    Figure Legend Snippet: ILz:rhTRAIL showed the highest cell death inducing ability in TRAIL susceptible cells. Indicated cells were cultured onto a 96-well plate and treated with varying recombinant TRAIL proteins: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL. ( a ) HeLa, CT26, and B16F10 cells were treated with varying recombinant TRAIL proteins (100 ng/ml). After 16 h, cell survival was examined by XTT assay. ( b ) Three human cell lines (Jurkat, MDA-MB-231, and HEK 293) and two murine cell lines (BMK and 4 T1) were treated with varying recombinant TRAIL proteins (100 ng/ml). To investigate TRAIL-susceptibility, cell death was analyzed by XTT assay 24 h after treatment. The relative values of the XTT assay were examined after comparing the results to untreated controls. * p < 0.05, compared with untreated controls by Student’s t -test

    Techniques Used: Cell Culture, Recombinant, XTT Assay

    ILz:rhTRAIL showed the highest cell death inducing ability in the combination treatment of recombinant TRAIL and bortezomib. Cells were cultured onto 96-well plates with 80% to 90% of confluency. Cell death rates were analyzed by XTT assay 24 h after the indicated treatment. The relative values of the XTT assay were examined following comparison to untreated controls. ( a ) Each cell was treated with 100 ng/ml of recombinant TRAIL protein and a distinctive amount of bortezomib as indicated: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL (ILz:rhTRAIL). ( b ) Two TRAIL-resistant cell lines (B16F10 and CT26) were treated with fixed amounts of bortezomib (50 and 100 nM) and serially increasing amounts of ILz:rhTRAIL. ( c ) Two TRAIL-sensitive cell lines (HEK 293 and MDA-MB-231) were treated with fixed amounts of bortezomib (25 and 50 nM) and serially increasing amounts of ILz:rhTRAIL: bort, bortezomib. * and ** p < 0.05 and p < 0.1 by Student’s t -test, respectively
    Figure Legend Snippet: ILz:rhTRAIL showed the highest cell death inducing ability in the combination treatment of recombinant TRAIL and bortezomib. Cells were cultured onto 96-well plates with 80% to 90% of confluency. Cell death rates were analyzed by XTT assay 24 h after the indicated treatment. The relative values of the XTT assay were examined following comparison to untreated controls. ( a ) Each cell was treated with 100 ng/ml of recombinant TRAIL protein and a distinctive amount of bortezomib as indicated: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL (ILz:rhTRAIL). ( b ) Two TRAIL-resistant cell lines (B16F10 and CT26) were treated with fixed amounts of bortezomib (50 and 100 nM) and serially increasing amounts of ILz:rhTRAIL. ( c ) Two TRAIL-sensitive cell lines (HEK 293 and MDA-MB-231) were treated with fixed amounts of bortezomib (25 and 50 nM) and serially increasing amounts of ILz:rhTRAIL: bort, bortezomib. * and ** p < 0.05 and p < 0.1 by Student’s t -test, respectively

    Techniques Used: Recombinant, Cell Culture, XTT Assay, Comparison

    Combined treatment-induced cell death was inhibited by anti-TRAIL antibody and pan-caspase inhibitor. ( a ) After the pre-treatment of anti-TRAIL antibody (1 μg/ml) for 1 h, B16F10 and CT26 cells were treated with the indicated amount of bortezomib (50 nM) and varying amounts of ILz:rhTRAIL: ILz:T, ILz:rhTRAIL; bort, bortezomib; anti-T, anti-TRAIL antibody. Cell death was analyzed by XTT assay 24 h after treatment. ( b ) B16F10 and CT26 cells were cultured onto a 96-well plate and treated with ILz:rhTRAIL (100 ng/ml) and bortezomib (100 nM) with or without z-VAD-fmk (50 μM) pre-treatment for 1 h. After 24 h, cell death was assayed by XTT: ILz:T, ILz:rhTRAIL; bort, bortezomib; z-VAD, z-VAD-fmk. * p < 0.05 by Student’s t -test. ( c ) B16F10 cells were stained with propidium iodide (PI) and Annexin V using FITC Annexin V Apoptosis Detection Kit (BD Biosciences). Cells were harvested after trypsin treatment and the stained populations were analyzed by Flow cytometry (FACSCalibur™, BD Biosciences, US) using BD CellQuest™ program. The populations (%) are marked in the figures
    Figure Legend Snippet: Combined treatment-induced cell death was inhibited by anti-TRAIL antibody and pan-caspase inhibitor. ( a ) After the pre-treatment of anti-TRAIL antibody (1 μg/ml) for 1 h, B16F10 and CT26 cells were treated with the indicated amount of bortezomib (50 nM) and varying amounts of ILz:rhTRAIL: ILz:T, ILz:rhTRAIL; bort, bortezomib; anti-T, anti-TRAIL antibody. Cell death was analyzed by XTT assay 24 h after treatment. ( b ) B16F10 and CT26 cells were cultured onto a 96-well plate and treated with ILz:rhTRAIL (100 ng/ml) and bortezomib (100 nM) with or without z-VAD-fmk (50 μM) pre-treatment for 1 h. After 24 h, cell death was assayed by XTT: ILz:T, ILz:rhTRAIL; bort, bortezomib; z-VAD, z-VAD-fmk. * p < 0.05 by Student’s t -test. ( c ) B16F10 cells were stained with propidium iodide (PI) and Annexin V using FITC Annexin V Apoptosis Detection Kit (BD Biosciences). Cells were harvested after trypsin treatment and the stained populations were analyzed by Flow cytometry (FACSCalibur™, BD Biosciences, US) using BD CellQuest™ program. The populations (%) are marked in the figures

    Techniques Used: XTT Assay, Cell Culture, Staining, Flow Cytometry



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    recombinant mouse trail protein rmtrail  (PeproTech)
    90
    PeproTech recombinant mouse trail protein rmtrail
    ILz:rhTRAIL showed the highest cell death inducing ability in <t>TRAIL</t> susceptible cells. Indicated cells were cultured onto a 96-well plate and treated with <t>varying</t> <t>recombinant</t> TRAIL proteins: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL. ( a ) HeLa, CT26, and B16F10 cells were treated with varying recombinant TRAIL proteins (100 ng/ml). After 16 h, cell survival was examined by XTT assay. ( b ) Three human cell lines (Jurkat, MDA-MB-231, and HEK 293) and two murine cell lines (BMK and 4 T1) were treated with varying recombinant TRAIL proteins (100 ng/ml). To investigate TRAIL-susceptibility, cell death was analyzed by XTT assay 24 h after treatment. The relative values of the XTT assay were examined after comparing the results to untreated controls. * p < 0.05, compared with untreated controls by Student’s t -test
    Recombinant Mouse Trail Protein Rmtrail, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+trail+protein+rmtrail/pmc05902847-58-3-12?v=PeproTech
    Average 90 stars, based on 1 article reviews
    recombinant mouse trail protein rmtrail - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ILz:rhTRAIL showed the highest cell death inducing ability in TRAIL susceptible cells. Indicated cells were cultured onto a 96-well plate and treated with varying recombinant TRAIL proteins: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL. ( a ) HeLa, CT26, and B16F10 cells were treated with varying recombinant TRAIL proteins (100 ng/ml). After 16 h, cell survival was examined by XTT assay. ( b ) Three human cell lines (Jurkat, MDA-MB-231, and HEK 293) and two murine cell lines (BMK and 4 T1) were treated with varying recombinant TRAIL proteins (100 ng/ml). To investigate TRAIL-susceptibility, cell death was analyzed by XTT assay 24 h after treatment. The relative values of the XTT assay were examined after comparing the results to untreated controls. * p < 0.05, compared with untreated controls by Student’s t -test

    Journal: BMC Cancer

    Article Title: The regulation of combined treatment-induced cell death with recombinant TRAIL and bortezomib through TRAIL signaling in TRAIL-resistant cells

    doi: 10.1186/s12885-018-4352-3

    Figure Lengend Snippet: ILz:rhTRAIL showed the highest cell death inducing ability in TRAIL susceptible cells. Indicated cells were cultured onto a 96-well plate and treated with varying recombinant TRAIL proteins: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL. ( a ) HeLa, CT26, and B16F10 cells were treated with varying recombinant TRAIL proteins (100 ng/ml). After 16 h, cell survival was examined by XTT assay. ( b ) Three human cell lines (Jurkat, MDA-MB-231, and HEK 293) and two murine cell lines (BMK and 4 T1) were treated with varying recombinant TRAIL proteins (100 ng/ml). To investigate TRAIL-susceptibility, cell death was analyzed by XTT assay 24 h after treatment. The relative values of the XTT assay were examined after comparing the results to untreated controls. * p < 0.05, compared with untreated controls by Student’s t -test

    Article Snippet: Recombinant human and mouse TRAIL proteins (rhTRAIL and rmTRAIL) were purchased from PeproTech (PeproTech Korea, Seoul, South Korea): rhTRAIL (310–04); rmTRAIL (315–19).

    Techniques: Cell Culture, Recombinant, XTT Assay

    ILz:rhTRAIL showed the highest cell death inducing ability in the combination treatment of recombinant TRAIL and bortezomib. Cells were cultured onto 96-well plates with 80% to 90% of confluency. Cell death rates were analyzed by XTT assay 24 h after the indicated treatment. The relative values of the XTT assay were examined following comparison to untreated controls. ( a ) Each cell was treated with 100 ng/ml of recombinant TRAIL protein and a distinctive amount of bortezomib as indicated: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL (ILz:rhTRAIL). ( b ) Two TRAIL-resistant cell lines (B16F10 and CT26) were treated with fixed amounts of bortezomib (50 and 100 nM) and serially increasing amounts of ILz:rhTRAIL. ( c ) Two TRAIL-sensitive cell lines (HEK 293 and MDA-MB-231) were treated with fixed amounts of bortezomib (25 and 50 nM) and serially increasing amounts of ILz:rhTRAIL: bort, bortezomib. * and ** p < 0.05 and p < 0.1 by Student’s t -test, respectively

    Journal: BMC Cancer

    Article Title: The regulation of combined treatment-induced cell death with recombinant TRAIL and bortezomib through TRAIL signaling in TRAIL-resistant cells

    doi: 10.1186/s12885-018-4352-3

    Figure Lengend Snippet: ILz:rhTRAIL showed the highest cell death inducing ability in the combination treatment of recombinant TRAIL and bortezomib. Cells were cultured onto 96-well plates with 80% to 90% of confluency. Cell death rates were analyzed by XTT assay 24 h after the indicated treatment. The relative values of the XTT assay were examined following comparison to untreated controls. ( a ) Each cell was treated with 100 ng/ml of recombinant TRAIL protein and a distinctive amount of bortezomib as indicated: rmT, recombinant mouse TRAIL from Peprotech (rmTRAIL); rhT, recombinant human TRAIL from Peprotech (rhTRAIL); ILz:T, isoleucine zipper hexamerization motif containing recombinant human TRAIL (ILz:rhTRAIL). ( b ) Two TRAIL-resistant cell lines (B16F10 and CT26) were treated with fixed amounts of bortezomib (50 and 100 nM) and serially increasing amounts of ILz:rhTRAIL. ( c ) Two TRAIL-sensitive cell lines (HEK 293 and MDA-MB-231) were treated with fixed amounts of bortezomib (25 and 50 nM) and serially increasing amounts of ILz:rhTRAIL: bort, bortezomib. * and ** p < 0.05 and p < 0.1 by Student’s t -test, respectively

    Article Snippet: Recombinant human and mouse TRAIL proteins (rhTRAIL and rmTRAIL) were purchased from PeproTech (PeproTech Korea, Seoul, South Korea): rhTRAIL (310–04); rmTRAIL (315–19).

    Techniques: Recombinant, Cell Culture, XTT Assay, Comparison

    Combined treatment-induced cell death was inhibited by anti-TRAIL antibody and pan-caspase inhibitor. ( a ) After the pre-treatment of anti-TRAIL antibody (1 μg/ml) for 1 h, B16F10 and CT26 cells were treated with the indicated amount of bortezomib (50 nM) and varying amounts of ILz:rhTRAIL: ILz:T, ILz:rhTRAIL; bort, bortezomib; anti-T, anti-TRAIL antibody. Cell death was analyzed by XTT assay 24 h after treatment. ( b ) B16F10 and CT26 cells were cultured onto a 96-well plate and treated with ILz:rhTRAIL (100 ng/ml) and bortezomib (100 nM) with or without z-VAD-fmk (50 μM) pre-treatment for 1 h. After 24 h, cell death was assayed by XTT: ILz:T, ILz:rhTRAIL; bort, bortezomib; z-VAD, z-VAD-fmk. * p < 0.05 by Student’s t -test. ( c ) B16F10 cells were stained with propidium iodide (PI) and Annexin V using FITC Annexin V Apoptosis Detection Kit (BD Biosciences). Cells were harvested after trypsin treatment and the stained populations were analyzed by Flow cytometry (FACSCalibur™, BD Biosciences, US) using BD CellQuest™ program. The populations (%) are marked in the figures

    Journal: BMC Cancer

    Article Title: The regulation of combined treatment-induced cell death with recombinant TRAIL and bortezomib through TRAIL signaling in TRAIL-resistant cells

    doi: 10.1186/s12885-018-4352-3

    Figure Lengend Snippet: Combined treatment-induced cell death was inhibited by anti-TRAIL antibody and pan-caspase inhibitor. ( a ) After the pre-treatment of anti-TRAIL antibody (1 μg/ml) for 1 h, B16F10 and CT26 cells were treated with the indicated amount of bortezomib (50 nM) and varying amounts of ILz:rhTRAIL: ILz:T, ILz:rhTRAIL; bort, bortezomib; anti-T, anti-TRAIL antibody. Cell death was analyzed by XTT assay 24 h after treatment. ( b ) B16F10 and CT26 cells were cultured onto a 96-well plate and treated with ILz:rhTRAIL (100 ng/ml) and bortezomib (100 nM) with or without z-VAD-fmk (50 μM) pre-treatment for 1 h. After 24 h, cell death was assayed by XTT: ILz:T, ILz:rhTRAIL; bort, bortezomib; z-VAD, z-VAD-fmk. * p < 0.05 by Student’s t -test. ( c ) B16F10 cells were stained with propidium iodide (PI) and Annexin V using FITC Annexin V Apoptosis Detection Kit (BD Biosciences). Cells were harvested after trypsin treatment and the stained populations were analyzed by Flow cytometry (FACSCalibur™, BD Biosciences, US) using BD CellQuest™ program. The populations (%) are marked in the figures

    Article Snippet: Recombinant human and mouse TRAIL proteins (rhTRAIL and rmTRAIL) were purchased from PeproTech (PeproTech Korea, Seoul, South Korea): rhTRAIL (310–04); rmTRAIL (315–19).

    Techniques: XTT Assay, Cell Culture, Staining, Flow Cytometry